In vitro and in vivo Propagation of Green Perilla (Perilla frutescens L. Britton var. crispa) Plants: A Comparative Study

Abstract

Author(s): Ely Zayova and Trendafil Nedev

An effective technique for rapid clonal micropropagation of Green perilla (Perilla
frutescens L. Britton var. crispa, Lamaiaceae) was established. High percentage of seed
stratification germination (95%) was recorded on Murashige and Skoog (MS) medium with 0.7%
agar and 3.0% sucrose after one weekof culture. The shoots were cultured on MS medium with 6-
Benzylaminopurine (BAP) and among the three auxin supplements: Indole-3-butyric acid (IBA); α-
Naphthalene acetic acid (NAA) or Indole-3-acetic acid (IAA) at concentration 0.1 mg l-1 after four
weeks of culture. The maximum number of shoots per explant (4.4) and shoot length (2.9 cm) gave
MS medium with 1.0 mg l-1BAP and 0.1 mg l-1 IAA. The best plant rooting was achieved on halfstrength
MS medium with 0.5 mg l-1 IBA after three weeks of culture. The multiple plants were
successfully ex vitro adapted with 95% survival after two months of ex vitro conditions.
Conventionally propagated plants by seeds was implemented, the persentage of seed germination
without stratification was 75%, while that of stratified seeds was higher and reached 100% after
one week of sowing.Some morphological characteristics as plant height, branches number per
plant, leaves number perplant, leaf size, flowers number on main, flowers number per plant and
seeds were measured after five months of field conditions. Different morphological features
between ex vitro and in vivo propaged plants were not established. This is the first comparative
study of micropropagated and in vivo seed derived perilla plants. The described protocol allows
the establishment of numerous micropropagated of P. frutescens plants.

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